Binding of six cephalosporins (cefotaxime, cefuroxime, cefazoline, cephalothin, cephaloridine, cephacetrile) to bovine serum albumin was studied. Fluorescence probe technique and difference spectrophotometry were employed to evaluate the nature and degree of association of cephalosporin-albumin complex. 1-Anilinonaphthalene-8-sulfonate (ANS) was used as the fluorescence probe. 2-(4¡¯¡¯-hydroxybenzeneazo) benzoic acid(HBAB) was employed as the UV spectrophotometric probe. Competitive bindings between cephalosporins and probes were observed. The number of binding sites of bovine serum albumin for each cephalosporin is 3. Among six cephalosporins, cefotaxime has the highest binding constant followed by cefazoline, cefuroxime, cephalothin, cephaloridine and cephacetrile.
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